Reproductive genomics mutagenesis strategy
The project uses a three-generation breeding scheme designed to select recessive mutations affecting reproductive functions. Initially, male C57BL6/J (B6) mice (Go generation) are treated with ENU and subsequently mated to C3HeB/FeJ (C3H) females to generate G1 offspring. Each of these G1 males is used to found a "family" that will segregate the unique set of mutations, some of which may affect reproduction. G1 males are mated to C3H females to produce G2 offspring. Females from the G2 population, each having a 50% chance of carrying any particular mutation from their G1 father, are mated back to the G1 father to generate the G3 population. In families segregating genetic mutations, each G3 animal has a 25% chance of being homozygous for a given mutant allele.
